High Performance Counter Current Chromatography (HPCCC) instruments are liquid-liquid chromatography devices that use partitioning between two immiscible liquid phases, where selectivity rather than efficiency is the main driver of resolution, to perform high resolution separations of a wide range of molecule types.

HPCCC is a preparative chromatography technique that chromatographers who use solid phase liquid chromatography techniques such as Prep-HPLC, flash or SFC, should consider. HPCCC instruments fit seamlessly into existing workflows and provide significant additional purification capability because of the following benefits:

Dynamic Extractions have fully equipped laboratories in the UK and USA, able to demonstrate the unique capabilities that HPCCC instruments can provide prospective customers and offer a contract separation service for those companies interested in cost effectively outsourcing their preparative chromatography projects.

Dynamic Extractions Ltd is a UK manufacturer, offering instruments from laboratory scale (milligram to 10s of grams) to pilot/manufacturing scales (kilo) and is the sole supplier of HPCCC instruments.

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Orthogonal Preparative Liquid Chromatography

High Performance Counter Current Chromatography (HPCCC) is an innovative, preparative-scale, liquid-liquid chromatographic technique. It is complementary and orthogonal to its solid-liquid 'conventional' chromatography counterparts - preparative hplc, reversed or normal phase, SFC or flash chromatography

In HPCCC, the stationary and mobile phases comprise a pair of selected immiscible liquids. Using liquids for both stationary and mobile phases confers a number of unique features for the preparative scale chromatographer:

Dynamic Extractions' HPCCC instruments (please see illustration contained installation page) replace the conventional, solid-packed column in standard HPLC set ups. High Performance Counter Current Chromatography is readily automated to provide scouting of purification methods in a manner very similar to that used in solid phase chromatographic method scouting.

Demonstrating the HPCCC advantage

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Dynamic Extractions has fully functional applications laboratories at both our UK headquarters in Slough, Berkshire and our USA headquarters in Monmouth Junction, NJ, at both of these facilities we are pleased to receive customer samples to perform feasibility studies, instrument demonstrations and outsourced contract separations. Typical separation work performed:

  • Purifications of milligram, gram and kilogram quantities of reference compounds, intermediates, drug candidates, analytical standards, metabolites, API's.
  • HPCCC method development using normal and reverse phase, elution-extrusion and pH zone refining conditions
  • Isolation of minor components of interest from complex mixtures

Interested customers should contact a member of the team by calling us on +44(0) 1753 696 979 or by using our simple contact us form.

Our products and solutions

Read about the key benefits of using liquid stationary phase and our High Performance Counter Current Chromatography Technologies.