Maximising both purity and recovery in preparative chromatography

High Performance Counter Current Chromatography (HPCCC) instruments allow you to add an orthogonal and complementary liquid chromatographic technique to your laboratory without major changes to your current workflows. In contrast to solid phase techniques, such as reverse or normal phase liquid chromatography (prep-HPLC) or SFC, the HPCCC stationary phase is a liquid rather than a solid. High resolution separations are possible due only to differences in the liquid-liquid partitioning behavior of sample solutes. Separations are dependent on improving selectivity, a powerful alternative to increasing efficiency when tackling difficult chromatographic separation problems. Preparative scale (mg to kg) chromatographers who use conventional chromatography techniques should consider using this technique which will fit seamlessly into workflows and provide productivity improvements in many applications.

Counter current chromatography is a high recovery technique which is as easy as the chromatography you do today but produces much higher yields and can use between a fifth and a tenth of the solvent usage. Request more information or a quotation now or Call us on +44(0) 1753 696 979.

HPCCC in use

Potential for Application of CCC in Chemical Development by GSK

A Quick Introduction

A quick introduction on Liquid Liquid Chromatography.

Orthogonal Preparative Liquid Chromatography

High Performance Counter Current Chromatography (HPCCC) is an innovative, preparative-scale, liquid-liquid chromatographic technique. It is complementary and orthogonal to its solid-liquid 'conventional' chromatography counterparts - preparative hplc, reversed or normal phase, SFC or flash chromatography

In HPCCC, the stationary and mobile phases comprise a pair of selected immiscible liquids. Using liquids for both stationary and mobile phases confers a number of unique features for the preparative scale chromatographer:

Selectivity driven high resolution purification
Significantly higher mass throughput
Simple and reliable scale-up
Greener chromatography

Dynamic Extractions' HPCCC instruments (please see illustration contained installation page) replace the conventional, solid-packed column in standard HPLC set ups. High Performance Counter Current Chromatography is readily automated to provide scouting of purification methods in a manner very similar to that used in solid phase chromatographic method scouting.

Demonstrating the HPCCC advantage

Dynamic Extractions has fully functional applications laboratories at both our UK headquarters in Slough, Berkshire and our USA headquarters in Monmouth Junction, NJ, at both of these facilities we are pleased to receive customer samples to perform feasibility studies, instrument demonstrations and outsourced contract separations. Typical separation work performed:

Purifications of milligram, gram and kilogram quantities of reference compounds, intermediates, drug candidates, analytical standards, metabolites, API's.
HPCCC method development using normal and reverse phase, elution-extrusion and pH zone refining conditions
Isolation of minor components of interest from complex mixtures

Interested customers should contact a member of the team by calling us on +44(0) 1753 696 979 or by using our simple contact us form.