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Simple quick HPCCC scale-up

Scaling up a purification process is essential when large quantities of materials are required. Solid-liquid chromatographic processes often require method redevelopment as purification scale increases. However, for HPCCC, load mass and volume-related dimensions scale linearly in proportion to the ratio of the column volumes being scaled.

The data below demonstrate a 42-fold scale up of the four component mixture comprising 1: Methyl 2-acetamido- 5-bromobenzoate; 2: Warfarin; 3: 4-Bromobenzamide; 4: Dipyridamole.

Analytical column
Loading 0.2 grams

Analytical column

Semi-prep column
Loading 1.2 grams

Semi-prep column

Preparative column
Loading 8.4 grams

Preparative column
Instrument Mini Spectrum Midi
Column analytical semi-preparative preparative
Column Volume (mL) 21 134 912
Nominal Scale Up Factor 1x 6x 42x
Flow Rate (mL/min) 1 6 42
Stationary Phase Retention (%) 84 89 88
Loading (g) 0.2 1.2 8.4
Loading Volume (mL) 2 12 82

Experimental Conditions:

HEMWat SS 17 (Hexane:Ethyl acetate:Methanol:Water,1:1:1:1, v/v/v/v), NP mode elution. In all three experiments, following 30 minutes of ‘classical' elution, the column contents were extruded by pumping LP through the column