Developing your Chromatography Purification Method

The key to all successful purifications is the development of the chromatographic method. In partition chromatography, two or more immiscible solvents might be used and therefore the potential choice of solvent systems is enormous. However, there are a number of common systems. The system generally considered first is Heptane / Ethyl Acetate / Methanol / Water; known as the HEMWat system, but there are a number of other common solvent series.

When performing a method development several members of a solvent series will be screened and the chromatography produced compared to see if the required target compound has been adequately resolved. The applications scientists at Dynamic Extractions laboratories, in the UK and USA, are available to offer advice on the most suitable solvent series for your particular compound.

These studies can be performed either manually or can be automated, if the necessary equipment is available. Dynamic Extractions is pioneering the use of on-demand solvent blending, as this leads to automated screening of solvent series, more accurately mixed solvent systems and reduced solvent usage, especially when producing 10s of millilitres of solvent system.