Integrated Purification – Separation Chromatography

Scale-up capability of high performance counter current chromatography

Dynamic Extractions is now able to offer the combination of the automated solvent system selection with the direct separation scale-up capability of high performance counter current chromatography instrumentation that provides a path to gram amounts of pure compound within a few hours. Reduced to actual practice, a few milligrams of a target sample is subjected to a standard scouting method, developed based upon the specific needs of the laboratory, covering perhaps 5-10 of the solvent systems.

Separation condition decisions are made by simple visual inspection of the resulting chromatographic data. The solvent combination scouting process is functionally equivalent to scouting multiple RP HPLC or SFC stationary phases. Once a solvent system has been selected, preparative scale separation is purely an issue of linear, volumetric scale up using a larger volume column on the HPCCC instrument. The chemist can complete this whole process within a day.

HPCCC Poster - Integrated liquid-liquid chromatography