Simple quick HPCCC scale-up

One of the key advantages that HPCCC products offer chromatographers in all fields is the ease with which purification, once developed, can be scaled to any required size.

First the method development is performed at the mg scale. Once the method has been developed and optimised, it can be quickly and simply scaled volumetrically to the required scale.

An example of volumetric scale-up is given in the table
to the right. As outlined above the method was
developed at mg scale and optimised for both volume
and concentration of sample injected; the results of
this work were then scaled up by the ratio of the column volume and then run at the kilo scale.

The chromatograms opposite show the effectiveness
of this method of scale-up. The reason for this is that HPCCC uses only one mechanism of purification. Once the purification method is developed, the columns are packed identically with stationary phase – because they are liquid – and the separations are run on HPCCC machines
that are designed to produce the same operating conditions regardless of the scale. By providing a range of equipment designed this way Dynamic Extractions makes it easy for the chemist to concentrate on their job – drug development; not chromatography development