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Chromatography Whitepapers

Download our selection of whitepapers, as listed below:

CounterCurrent Chromatography

CounterCurrent Chromatography: An Introduction

Benefits of Liquid Chromatography

Dynamic Extractions gives you the top benefits of using liquid chromatography.

Chromatography Elution Mode Control

When using SLC methodologies different columns can be used for varying experiment. Dynamic Extractions writes on the processes involved.

Chromatography pH Control

Dynamic Extractions Whitepapers Dynamic Extraction brings you a short intro to pH control and the effect it can have on selectivity within the chromatography process.

Chromatography Procedures for Experiments on Centrifugal LLC

Dynamic Extractions provides information on the standard procedures used when running centrifugal liquid chromatography experiments.

What Is High Performance Counter Current Chromatography

Why was the g force in hydrodynamic CCC machines limited to 80g? If 80g gave such a step-jump in improving SP retention and performance, what if the applied g force could be increased to offer the implementation of yet higher flow rates to effect even shorter experimental duration? Higher g forces require higher rotation speeds (or impractically large rotor radii) but this required the consequent and necessary dissipation of more generated heat.

The History of Counter Current Chromatography

Dynamic Extractions provides information on the history of chromatography. From the first CCC machine to the improvement of the process.

Counter Current Chromatography Resolution

Understanding how resolution is achieved in CCC is assisted by the use of the simple model of a series of separating funnels numbered F1 - F100, each containing, let's say 10ml of ethyl acetate which has been pre-saturated with water and which will form the SP for our experiments. For our first experiment, 10ml of water, pre-saturated with ethyl acetate and 50 parts of a solute with a D value of 1, when partitioned between ethyl acetate and water, are added to the first funnel. The funnel is stoppered, shaken and the phases allowed to settle. At this point each of the phases will contain 25 parts of the solute.

Chromatography Concepts and Principles

The technique of biphasic liquid-liquid extraction is very familiar to synthetic chemists as a general, simple and robust post-reaction work-up step. In its simplest form it depends upon arranging conditions such that reaction products, by-products and un-reacted reagents have either very high (approaching ∞) or very low (approaching 0) partition coefficients i.e. that these solutes will be very soluble in either the organic phase or the aqueous phase and virtually insoluble in the other. Typically, the procedure might be used following a reaction to couple an organic base and an organic acid to form an amide.